A novel role of DNA polymerase λ in translesion synthesis in conjunction with DNA polymerase ζ

The POLD3 subunit of DNA polymerase δ can promote translesion synthesis independently of DNA polymerase ζ

The replicative DNA polymerase Polδ consists of a catalytic subunit POLD1/p125 and three regulatory subunits POLD2/p50, POLD3/p66 and POLD4/p12. The ortholog of POLD3 in Saccharomyces cerevisiae, Pol32, is required for a significant proportion of spontaneous and UV-induced mutagenesis through its additional role in translesion synthesis (TLS) as a subunit of DNA polymerase ζ. Remarkably, chicke...

Biological role of Escherichia coli translesion synthesis DNA polymerase IV

Damage tolerance is a measure of last resort to rescue cells from DNA damage, without which cells would become highly sensitive to killing by DNA-damaging agents. DNA lesion can be tolerated via different pathways, of which two best studied are homologous recombination and replicative lesion bypass. Replicative lesion bypass requires specialized DNA polymerases, most of which belong to the Y-fa...

Eukaryotic DNA polymerase ζ.

This review focuses on eukaryotic DNA polymerase ζ (Pol ζ), the enzyme responsible for the bulk of mutagenesis in eukaryotic cells in response to DNA damage. Pol ζ is also responsible for a large portion of mutagenesis during normal cell growth, in response to spontaneous damage or to certain DNA structures and other blocks that stall DNA replication forks. Novel insights in mutagenesis have be...

Requirement of Rad5 for DNA polymerase ζ-dependent translesion synthesis in Saccharomyces cerevisiae

DNA Polymerase β Gap-Filling Translesion DNA Synthesis

Although the primary function of DNA polymerase (pol) β is associated with gap-filling DNA synthesis as part of the DNA base excision repair pathway, translesion synthesis activity has also been described. To further understand the potential role of pol β-catalyzed translesion DNA synthesis (TLS) and the structure-function relationships of specific residues in pol β, wild-type and selected muta...